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This is my first attempt at using a Canon 65mm f/2.8 1x-5x macro lens. I needed to get within 2" of a live spider sitting on his web. The abdomen is about 5/32" of an inch and the legs about as thick as fishing line. The lines in the background are the rest of the legs. The f-stop was set to about 30 to get this DOF. ISO set to 3200 and the shutter 1/15 second. I used 2500 watts of lighting which is almost useless at 2". I couldn't use a ring flash because it hit the web and the spider scampered away.
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